3K7X
Crystal structure of the Lin0763 protein from Listeria innocua. Northeast Structural Genomics Consortium Target LkR23.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97879 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.194, 75.333, 53.756 |
| Unit cell angles | 90.00, 95.28, 90.00 |
Refinement procedure
| Resolution | 33.400 - 1.889 |
| R-factor | 0.1683 |
| Rwork | 0.167 |
| R-free | 0.18430 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.174 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.889 | 1.900 |
| Rmerge | 0.109 | 0.418 |
| Number of reflections | 56453 | |
| <I/σ(I)> | 13.2 | 2.6 |
| Completeness [%] | 97.9 | 84.4 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER PARAFFIN OIL | 10 | 291 | 40% PEG 8000, 0.1M KH2PO4, 0.1M CAPS, pH 10.0, MICROBATCH UNDER PARAFFIN OIL, temperature 291K |
