3K7O
Structure of type B ribose 5-phosphate isomerase from Trypanosoma cruzi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 93.199, 93.199, 93.712 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k7p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.099 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.082 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.078 | 0.641 |
| Total number of observations | 20502 | |
| Number of reflections | 28418 | |
| <I/σ(I)> | 13.1 | 1.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.5% (v/v) Jeffamine ED-2001, 0.1M HEPES, 1.1 M Na-malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
