3K7N

Structures of two elapid snake venom metalloproteases with distinct activities highlight the disulfide patterns in the D domain of ADAMalysin family proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	110
Detector technology	CCD
Collection date	2007-09-28
Detector	ADSC QUANTUM 315
Spacegroup name	P 43 21 2
Unit cell lengths	62.999, 62.999, 273.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.235
Rwork	0.234
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k7l
RMSD bond length	0.029
RMSD bond angle	2.560
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	25427
Completeness [%]	99.3	100
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.8	293	10% PEG8000, 8% ETHYLENE GLYCOL, 0.1M HEPES, pH 6.8, temperature 293K
1		6.8	293	10% PEG8000, 8% ETHYLENE GLYCOL, 0.1M HEPES, pH 6.8, temperature 293K