3K7M

Crystal structure of 6-hydroxy-L-nicotine oxidase from Arthrobacter nicotinovorans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-23
Detector	MAR CCD 165 mm
Wavelength(s)	1.05
Spacegroup name	P 4 3 2
Unit cell lengths	163.919, 163.920, 163.919
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.400 - 1.950
R-factor	0.17796
Rwork	0.176
R-free	0.21340
Structure solution method	MAD
RMSD bond length	0.035
RMSD bond angle	2.630
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.059	0.623
Number of reflections	54917
<I/σ(I)>	28.9	3
Completeness [%]	99.6	94.3
Redundancy	15	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20mM Sodium phosphate, 4M sodium formiate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20mM Sodium phosphate, 4M sodium formiate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20mM Sodium phosphate, 4M sodium formiate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K