3K7L

Structures of two elapid snake venom metalloproteases with distinct activities highlight the disulfide patterns in the D domain of ADAMalysin family proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	110
Detector technology	CCD
Collection date	2006-07-18
Detector	ADSC QUANTUM 315
Spacegroup name	P 43 21 2
Unit cell lengths	91.650, 91.650, 124.235
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.225
Rwork	0.224
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ero
RMSD bond length	0.033
RMSD bond angle	2.750
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.480	2.480
Number of reflections	18724
<I/σ(I)>	43.8
Completeness [%]	96.6	100
Redundancy	19.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	7% PEG6000, 0.1M SODIUM CITRATE, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5	293	7% PEG6000, 0.1M SODIUM CITRATE, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K