3K7I
Crystal structure of the E131K mutant of the Indian Hedgehog N-terminal signalling domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-04-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.430, 53.190, 35.440 |
| Unit cell angles | 90.00, 91.39, 90.00 |
Refinement procedure
| Resolution | 21.653 - 1.438 |
| R-factor | 0.1638 |
| Rwork | 0.163 |
| R-free | 0.17850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vhh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.159 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.830 | 1.510 |
| High resolution limit [Å] | 1.438 | 1.438 |
| Rmerge | 0.044 | 0.169 |
| Number of reflections | 34957 | |
| <I/σ(I)> | 20.2 | 7.6 |
| Completeness [%] | 95.4 | 87.5 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1M sodium cacodylate, pH 6.5, 30% PEG 8000, 0.2M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
