3K7B
The structure of the poxvirus A33 protein reveals a dimer of unique C-type lectin-like domains.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.940, 56.320, 41.680 |
| Unit cell angles | 90.00, 110.68, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.048 | 0.304 |
| Number of reflections | 10073 | |
| <I/σ(I)> | 26.9 | 4.4 |
| Completeness [%] | 97.5 | 96.2 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 298 | 20% PEG 8000, 10% isopropanol, 0.1M Sodium Acetate, pH 4.30, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
