3K7A

Crystal Structure of an RNA polymerase II-TFIIB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	93
Detector technology	CCD
Collection date	2008-08-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97946
Spacegroup name	I 2 2 2
Unit cell lengths	204.150, 216.210, 420.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.800
R-factor	0.268
Rwork	0.263
R-free	0.31300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1R5U WITHOUT CHAIN M
RMSD bond length	0.013
RMSD bond angle	1.363
Data scaling software	SCALA (3.2.25)
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	210.490	149.070	4.010
High resolution limit [Å]	3.800	12.020	3.800
Rmerge	0.093	0.034	0.545
Rmeas		0.040	0.750
Rpim		0.020	0.370
Total number of observations		10790	50783
Number of reflections	89990
<I/σ(I)>	9.6	32.7	2
Completeness [%]	98.6	96.1	99.3
Redundancy	3.9	3.7	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	295	1.2 M sodium/potassium phosphate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K