3K73
Crystal Structure of Phosphate bound Holo Glyceraldehyde-3-phosphate dehydrogenase 1 from MRSA252 at 2.5 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.200, 104.851, 90.604 |
| Unit cell angles | 90.00, 107.57, 90.00 |
Refinement procedure
| Resolution | 19.140 - 2.500 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3H48 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.553 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.8L) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.380 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.085 | 0.376 |
| Total number of observations | 12030 | |
| Number of reflections | 41937 | |
| <I/σ(I)> | 8.1 | 2.5 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 3.02 | 2.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris-HCl pH 8.5, 25% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
