3K6M
Dynamic domains of Succinyl-CoA:3-ketoacid-coenzyme A transferase from pig heart.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-06-25 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.948 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.715, 133.566, 102.230 |
| Unit cell angles | 90.00, 104.98, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.1657 |
| Rwork | 0.165 |
| R-free | 0.18538 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o9l |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.528 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.042 | 0.296 |
| Number of reflections | 297163 | |
| <I/σ(I)> | 12 | 2.3 |
| Completeness [%] | 97.7 | 82 |
| Redundancy | 3.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 4 ul Well Solution: 18-22% PEG 3350 or 4000, 75 mM Tris/HCl pH 8. 4 ul Protein solution: 10-15 mg/ml protein, 20 mM MOPS pH 7.2, 1 mM EDTA, 1 mM DTT, 0.2 mM PMSF, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
