3K6J
Crystal structure of the dehydrogenase part of multifuctional enzyme 1 from C.elegans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 78.025, 90.657, 66.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.090 - 2.200 |
| R-factor | 0.21256 |
| Rwork | 0.210 |
| R-free | 0.26734 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zcj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.278 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.090 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.101 | 0.676 |
| Number of reflections | 24533 | |
| <I/σ(I)> | 26.6 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.9 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 0.1M Tris-HCl, 0.1M K2HPO4, 20% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
