3K6A
Crystal structure of molybdenum cofactor biosynthesis protein mog from shewanella oneidensis
Replaces: 2FUWExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97956 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.132, 66.310, 74.332 |
| Unit cell angles | 92.15, 103.64, 119.98 |
Refinement procedure
| Resolution | 50.000 - 1.890 |
| Rwork | 0.177 |
| R-free | 0.21633 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.890 | 1.900 |
| Rmerge | 0.106 | 0.237 |
| Number of reflections | 79669 | |
| <I/σ(I)> | 30.61 | 5.35 |
| Completeness [%] | 96.8 | 94.7 |
| Redundancy | 4.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 0.1 M K THIOCYANATE 30 % PEGMME 2000, PH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
