3K63
X-ray structure of the PF04200 domain from Q9PRA0_UREPA protein of Ureaplasma parvum. NESG target UuR17a.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.557, 60.557, 64.716 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.538 - 2.494 |
| R-factor | 0.2147 |
| Rwork | 0.213 |
| R-free | 0.24800 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.639 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX (D/E) |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.494 | 2.500 |
| Rmerge | 0.089 | 0.812 |
| Number of reflections | 9252 | |
| <I/σ(I)> | 36.8 | 3.65 |
| Completeness [%] | 99.5 | 97.9 |
| Redundancy | 11.2 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER PARAFFIN OIL | 7.5 | 291 | 40% PEG 8000, 0.1M KBr, 0.1M HEPES pH 7.5, MICROBATCH UNDER PARAFFIN OIL, temperature 291K |
