3K62

Crystal structure of FBF-2/gld-1 FBEb complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 61
Unit cell lengths	96.620, 96.620, 101.678
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.892 - 1.900
Rwork	0.158
R-free	0.19730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k5q
RMSD bond length	0.006
RMSD bond angle	0.722
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.892	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	42208
<I/σ(I)>	28.4	2.9
Completeness [%]	99.6	93.5
Redundancy	11.2	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM Tris pH 7.5, 10% polyethylene glycol 8000, and 8% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K