3K5Z

Crystal structure of FBF-2/gld-1 FBEa G4A mutant complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 61
Unit cell lengths	97.048, 97.048, 101.703
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.015 - 2.400
Rwork	0.160
R-free	0.23070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k5q
RMSD bond length	0.006
RMSD bond angle	0.731
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.015	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	21281
<I/σ(I)>	18.2	3.7
Completeness [%]	99.8	99.4
Redundancy	8.7	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM Tris pH 7.5, 10% polyethylene glycol 8000, and 8% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K