3K5B
Crystal structure of the peripheral stalk of Thermus thermophilus H+-ATPase/synthase
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97957 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.781, 79.755, 75.796 |
| Unit cell angles | 90.00, 97.64, 90.00 |
Refinement procedure
| Resolution | 29.660 - 3.100 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.28800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.910 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 15485 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 291 | 40% MPD, 0.1 M Cacodylate pH 6.4, 5% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
