3K4T
Crystal structure of the virion-associated protein P3 from caulimovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-20 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.302, 28.818, 75.957 |
| Unit cell angles | 90.00, 92.08, 90.00 |
Refinement procedure
| Resolution | 18.980 - 2.590 |
| R-factor | 0.223 |
| Rwork | 0.219 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f6n |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.255 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.980 | 2.740 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.074 | 0.302 |
| Number of reflections | 9317 | |
| <I/σ(I)> | 14.4 | 3.6 |
| Completeness [%] | 96.1 | 99.2 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 291 | 25% PEG 1000, 0.1M MES-NAOH BUFFER, 1.2 MOLAR-EXCESS DNA OLIGONUCLEOTIDE (POLY-AT, 14 BP) , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
