3K4E
Puf3 RNA binding domain bound to Cox17 RNA 3' UTR recognition sequence site A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-12 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.078, 87.134, 124.910 |
| Unit cell angles | 90.00, 116.23, 90.00 |
Refinement procedure
| Resolution | 28.910 - 3.200 |
| R-factor | 0.252 |
| Rwork | 0.247 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k49 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.840 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.153 | 0.453 |
| Number of reflections | 23487 | |
| <I/σ(I)> | 8.6 | 2.8 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 277 | 20% PEG4000, 0.1 M sodium citrate pH 4.8, 2% dextran sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
