3K49
Puf3 RNA binding domain bound to Cox17 RNA 3' UTR recognition sequence site B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.126, 87.071, 125.176 |
| Unit cell angles | 90.00, 116.48, 90.00 |
Refinement procedure
| Resolution | 36.610 - 2.500 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m8y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.952 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.104 | 0.351 |
| Number of reflections | 48055 | |
| <I/σ(I)> | 12.4 | 3.5 |
| Completeness [%] | 94.2 | 83.4 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 277 | 20% PEG4000, 0.1 M sodium citrate pH4.8, 2% dextran sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
