3K3N
Crystal structure of the catalytic core domain of human PHF8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-05-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97939 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.238, 52.583, 134.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yu2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.019 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.055 | 0.426 |
| Number of reflections | 14988 | |
| <I/σ(I)> | 31 | 2.6 |
| Completeness [%] | 98.0 | 4.1 |
| Redundancy | 5.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 200mM KCl, 100mM Mes, 20% PEG5K, pH 6.5, VAPOR DIFFUSION, temperature 298K |
