3K3K
Crystal structure of dimeric abscisic acid (ABA) receptor pyrabactin resistance 1 (PYR1) with ABA-bound closed-lid and ABA-free open-lid subunits
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115932 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 50.208, 61.407, 82.844 |
| Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f08 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.512 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.054 | 0.391 |
| Number of reflections | 53191 | |
| <I/σ(I)> | 22.8 | 2.5 |
| Completeness [%] | 96.6 | 76.6 |
| Redundancy | 3.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.2 | 293 | 20% PEG MME 2000, 5% Ammonium sulfate, 5% NaCl, pH 6.2, VAPOR DIFFUSION, temperature 293K |
