3K32
The crystal structure of predicted subunit of tRNA methyltransferase from Methanocaldococcus jannaschii DSM
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-20 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97929, 0.97948 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.669, 121.951, 81.224 |
| Unit cell angles | 90.00, 116.70, 90.00 |
Refinement procedure
| Resolution | 34.775 - 2.500 |
| R-factor | 0.1813 |
| Rwork | 0.177 |
| R-free | 0.26310 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.102 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.101 | 0.398 |
| Number of reflections | 51681 | |
| <I/σ(I)> | 13.35 | 1.94 |
| Completeness [%] | 97.8 | 95.3 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1M Bistris Propane 7.0, 0.6M K/Na tartrate tetrahydrated, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
