3K30
Histamine dehydrogenase from Nocardiodes simplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.141, 107.035, 153.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.430 - 2.700 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1djn |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.753 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.500 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 46309 | |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 9.7 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 293 | 0.1 HEPES buffer (pH 7.4) containing 2.0 M (NH4)2SO4 and 2% 400-PEG (v/v), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
