3K2Y

Crystal structure of protein lp_0118 from Lactobacillus plantarum,northeast structural genomics consortium target LpR91B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-02
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	51.254, 86.288, 110.512
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.500 - 2.400
R-factor	0.229
Rwork	0.229
R-free	0.27100
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.070	0.345
Number of reflections	37231
Completeness [%]	100.0	100
Redundancy	7.6	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	100 mM NH4H2PO4, 100mM naCitrate Ph 4.2, 24% PEG 20K, VAPOR DIFFUSION, SITTING DROP, temperature 293K