3K2N
The crystal structure of sigma-54-dependent transcriptional regulator domain from Chlorobium Tepidum TLS
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-18 |
Detector | SBC-2 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.121, 81.250, 83.522 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.615 - 2.500 |
R-factor | 0.2009 |
Rwork | 0.197 |
R-free | 0.28030 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.173 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.660 |
High resolution limit [Å] | 2.500 | 2.580 |
Rmerge | 0.103 | 0.297 |
Number of reflections | 16911 | |
<I/σ(I)> | 4.875 | 6.9 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 8.9 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2M Na acetate, 20% PEG3k, 0.1M Na-Hepes PH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |