3K2D

Crystal structure of Immunogenic lipoprotein A from Vibrio vulnificus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-02
Detector	ADSC QUANTUM 210
Wavelength(s)	1.23984
Spacegroup name	F 4 3 2
Unit cell lengths	273.600, 273.600, 273.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.600
Rwork	0.225
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.370
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.137	0.563
Number of reflections	25307
<I/σ(I)>	38.4	15
Completeness [%]	100.0	100
Redundancy	82	77.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.1M HEPES, 2.25M ammonium sulfate, 3% sorbitol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.1M HEPES, 2.25M ammonium sulfate, 3% sorbitol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K