3K2A

Crystal structure of the homeobox domain of human homeobox protein Meis2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-05
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97910
Spacegroup name	I 4 2 2
Unit cell lengths	113.597, 113.597, 50.251
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.130 - 1.950
R-factor	0.216
Rwork	0.215
R-free	0.23900
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.082
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.020
High resolution limit [Å]	1.950	4.200	1.950
Rmerge	0.063	0.047	0.473
Number of reflections	12319
<I/σ(I)>	17.6
Completeness [%]	99.6	96.5	100
Redundancy	28.5	25.8	29

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	28% PEG4000, 8% isopropanol, 0.1M sodium acetate, 10mM L-proline, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K