3K1B
Structure of OmpF porin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 215.529, 215.529, 137.465 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 4.390 |
| R-factor | 0.26695 |
| Rwork | 0.264 |
| R-free | 0.32887 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2omf |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.857 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.480 |
| High resolution limit [Å] | 4.400 | 4.400 |
| Rmerge | 0.211 | 0.512 |
| Number of reflections | 23572 | |
| <I/σ(I)> | 7.778 | 3 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 5.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 3.9 | 293 | 0.48 sodium phosphate monobasic, 0.72 M potassium phosphate monobasic, 0.1 M acetate pH 3.9, VAPOR DIFFUSION, temperature 293K |
