3K10

Crystal structure of telomere capping protein Stn1 from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	93.15
Detector technology	IMAGE PLATE
Collection date	2008-08-04
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.502
Spacegroup name	P 43 21 2
Unit cell lengths	52.709, 52.709, 186.552
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
Rwork	0.246
R-free	0.30100
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	1.320
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.13)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.063	0.032	0.421
Number of reflections	13389
<I/σ(I)>	14.3
Completeness [%]	85.5	99.9	35.5
Redundancy	8.6	9.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	290	15% w/v PEG 1000, 10 mM EDTA, 0.1 M Tris-HCl pH 8.4, 10mM PtCl4, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	8.4	290	15% w/v PEG 1000, 10 mM EDTA, 0.1 M Tris-HCl pH 8.4, 10mM PtCl4, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	8.4	290	15% w/v PEG 1000, 10 mM EDTA, 0.1 M Tris-HCl pH 8.4, 10mM PtCl4, VAPOR DIFFUSION, HANGING DROP, temperature 290K