3K0X
Crystal structure of telomere capping protein Ten1 from Saccharomyces pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93.15 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.502 |
| Spacegroup name | H 3 |
| Unit cell lengths | 64.304, 64.304, 66.242 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| Rwork | 0.188 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.016 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.12) |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 22.080 | 22.080 | 1.790 |
| High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
| Rmerge | 0.027 | 0.336 | |
| Total number of observations | 1755 | 3061 | |
| Number of reflections | 10634 | ||
| <I/σ(I)> | 10.205 | 17.1 | 2.2 |
| Completeness [%] | 94.5 | 98.8 | 69.5 |
| Redundancy | 4.5 | 5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 290 | 0.2 M Potassium nitrate, 25% PEG 3350, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
