3K0X

Crystal structure of telomere capping protein Ten1 from Saccharomyces pombe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	93.15
Detector technology	IMAGE PLATE
Collection date	2009-05-08
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.502
Spacegroup name	H 3
Unit cell lengths	64.304, 64.304, 66.242
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.700
Rwork	0.188
R-free	0.23800
Structure solution method	SAD
RMSD bond length	0.021
RMSD bond angle	2.016
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.12)
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.080	22.080	1.790
High resolution limit [Å]	1.700	5.380	1.700
Rmerge		0.027	0.336
Total number of observations		1755	3061
Number of reflections	10634
<I/σ(I)>	10.205	17.1	2.2
Completeness [%]	94.5	98.8	69.5
Redundancy	4.5	5	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	290	0.2 M Potassium nitrate, 25% PEG 3350, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K