3K0V
Removal of sugars and sugars-like molecules from the solution by C-lobe of lactoferrin: Crystal structure of the complex of C-lobe with beta-D-glucopyranosyl-(1->4)-beta-D-galactopyranosyl-(1->4)-alpha-D-glucopyranose at 1.9 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.810, 50.134, 65.545 |
| Unit cell angles | 90.00, 107.10, 90.00 |
Refinement procedure
| Resolution | 62.000 - 1.910 |
| R-factor | 0.20884 |
| Rwork | 0.207 |
| R-free | 0.23685 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ibo |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.620 | 1.960 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 29538 | |
| <I/σ(I)> | 7.1 | 2.5 |
| Completeness [%] | 96.0 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.01M Znso4, 0.1M MES, 25% PEG, Monomethyl Ether 550, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
