3K0M
Cryogenic structure of CypA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.953695 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.419, 51.741, 88.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.659 - 1.250 |
| R-factor | 0.1305 |
| Rwork | 0.129 |
| R-free | 0.14920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cpl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.307 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.4_162)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 54771 | |
| <I/σ(I)> | 38.3 | 3.6 |
| Completeness [%] | 96.1 | 89.8 |
| Redundancy | 7.4 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 100 mM HEPES pH 7.5, 22% PEG 3350, 5 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
