3K0I
Crystal structure of Cu(I)CusA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.3779 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 179.256, 179.256, 286.266 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.458 - 4.116 |
| R-factor | 0.2705 |
| Rwork | 0.269 |
| R-free | 0.30850 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.248 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.270 |
| High resolution limit [Å] | 4.116 | 4.116 |
| Rmerge | 0.089 | 0.457 |
| Number of reflections | 26090 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 98.1 | 99.5 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% PEG 3350, 0.1M Na citrate, 0.5M (NH4)2SO4, 10% Glycerol, 1% JM600 pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
