3K0A
Crystal structure of the phosphorylation-site mutant S431A of the KaiC circadian clock protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 113.15 |
| Detector technology | CCD |
| Collection date | 2007-11-07 |
| Detector | MAR 300MM CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 133.230, 134.960, 204.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbl |
| Data reduction software | HKL-2000 |
| Data scaling software | XSCALE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.000 | 3.200 |
| Rmerge | 0.078 | 0.085 |
| Number of reflections | 62726 | |
| <I/σ(I)> | 10.7 | 1 |
| Completeness [%] | 84.6 | 37.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 291 | SODIUM FORMATE, GLYCEROL, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
