3JZY

Crystal structure of human Intersectin 2 C2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97711
Spacegroup name	I 41 2 2
Unit cell lengths	54.125, 54.125, 196.709
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.560
R-factor	0.223
Rwork	0.222
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ep6
RMSD bond length	0.016
RMSD bond angle	1.607
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.590
High resolution limit [Å]	1.560	4.230	1.560
Rmerge	0.079	0.045	0.890
Number of reflections	21447
<I/σ(I)>	13.4
Completeness [%]	99.9	97.9	100
Redundancy	17.7	15.7	15.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	20% PEG3350, 0.2M calcium chloride. calcium chloride was also added to the protein stock solution to a final concentration of 0.002M. Crystals were detected only after more than 100 days of incubation., vapor diffusion, temperature 291K