3JZT
Structure of a cubic crystal form of X (ADRP) domain from FCoV with ADP-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.812 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 220.236, 220.236, 220.236 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 3.910 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.601 |
| Data reduction software | COMBAT |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.130 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.463 | 0.670 |
| Number of reflections | 32246 | |
| <I/σ(I)> | 4.45 | 2.5 |
| Completeness [%] | 98.9 | 98.5 |
| Redundancy | 7.5 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 298 | di-ammonium citrate, pH 6.0, VAPOR DIFFUSION, temperature 298K |
