3JZ9

Crystal structure of the GEF domain of DrrA/SidM from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.007490
Spacegroup name	P 62
Unit cell lengths	126.338, 126.338, 35.925
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.350 - 1.800
R-factor	0.17
Rwork	0.169
R-free	0.19800
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.034
RMSD bond angle	2.304
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC (5.5.0070)
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.900
High resolution limit [Å]	1.800	25.000	1.800
Rmerge	0.059	0.023	0.462
Number of reflections	30729	6	4518
<I/σ(I)>	21.28	81.9	4
Completeness [%]	99.7	100	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.25 M Sodium sulfate, 21% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 292K