3JZ3

Structure of the cytoplasmic segment of histidine kinase QseC

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	77
Detector technology	CCD
Collection date	2008-08-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.96789
Spacegroup name	P 41 21 2
Unit cell lengths	70.737, 70.737, 176.573
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.690 - 2.500
R-factor	0.22996
Rwork	0.228
R-free	0.26234
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	0.909
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge		0.294
Number of reflections	16317
<I/σ(I)>		10.4
Completeness [%]	99.9	100
Redundancy		13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	8-10 mg/ml protein with 27-30% (NH4)2SO4, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	8-10 mg/ml protein with 27-30% (NH4)2SO4, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K