3JYZ

Crystal structure of Pseudomonas aeruginosa (strain: Pa110594) typeIV pilin in space group P41212

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-12
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9800
Spacegroup name	P 41 21 2
Unit cell lengths	63.059, 63.059, 67.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.842 - 1.550
R-factor	0.1801
Rwork	0.177
R-free	0.20880
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.391
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.550
Rmerge	0.068
Number of reflections	20404
<I/σ(I)>	33.9
Completeness [%]	100.0
Redundancy	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	0.1 M Tris-HCl pH 8.5, 2.0 M Ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 294K