3JYU

Crystal structure of the N-terminal domains of the ubiquitin specific peptidase 4 (USP4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-21
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97942
Spacegroup name	C 1 2 1
Unit cell lengths	180.060, 34.060, 125.190
Unit cell angles	90.00, 133.52, 90.00

Refinement procedure

Resolution	41.227 - 2.370
R-factor	0.1844
Rwork	0.182
R-free	0.23340
Structure solution method	MRSAD
Starting model (for MR)	1w6v
RMSD bond length	0.008
RMSD bond angle	1.062
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.4_138))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.400	2.500
High resolution limit [Å]	2.370	2.370
Rmerge	0.096	0.349
Number of reflections	22745
<I/σ(I)>	11.9	4.1
Completeness [%]	99.1	98.4
Redundancy	6.9	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	291	1 volume of 9 mg/ml protein solution was mixed with 1 volume of a solution of 20% (w/v) PEG 4000, 10% (v/v) isopropanool, 0.1 M sodium HEPES, and 0.001 M dithiothreitol and equilibrated against 1 M NaCl (well solution). The crystal was cryoprotected in well solution mixed 1:1 with a solution containing 60% (v/v) ethylene glycol and 200 mg/ml 3-(1-pyridino)-propene sulfonate (NDSB-201) and frozen by immersion in liquid nitrogen, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K