3JYS

Crystal structure of SusD superfamily protein (YP_001298690.1) from Bacteroides vulgatus ATCC 8482 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97936
Spacegroup name	P 41 21 2
Unit cell lengths	63.929, 63.929, 266.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.111 - 2.000
R-factor	0.179
Rwork	0.178
R-free	0.19800
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.476
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.111	29.600	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.075	0.036	0.643
Total number of observations		2956	20230
Number of reflections	38690
<I/σ(I)>	16.8	16.7	1.2
Completeness [%]	100.0	96.7	100
Redundancy	7.1	5.6	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	277	0.2000M MgCl2, 20.0000% PEG-3350, No Buffer pH 5.8, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K