3JYR
Crystal structures of the GacH receptor of Streptomyces glaucescens GLA.O in the unliganded form and in complex with acarbose and an acarbose homolog. Comparison with acarbose-loaded maltose binding protein of Salmonella typhimurium.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.921, 89.229, 64.163 |
| Unit cell angles | 90.00, 112.77, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.750 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.281 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.130 | 40.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.043 | 0.041 | 0.120 |
| Number of reflections | 37653 | ||
| <I/σ(I)> | 22.6 | ||
| Completeness [%] | 93.4 | 99.9 | 64.6 |
| Redundancy | 3.6 | 3.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 18% PEG 8000, 10 mM CITRATE, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
