3JYM

Crystal Structure of the 3 FKBP domains of wheat FKBP73

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-05
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9334
Spacegroup name	P 1 21 1
Unit cell lengths	186.956, 31.012, 68.978
Unit cell angles	90.00, 93.59, 90.00

Refinement procedure

Resolution	46.630 - 2.280
R-factor	0.27355
Rwork	0.270
R-free	0.34327
Structure solution method	PHASER
RMSD bond length	0.035
RMSD bond angle	2.810
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.630	2.340
High resolution limit [Å]	2.280	2.280
Rmerge	0.077	0.484
Number of reflections	36745
<I/σ(I)>	21	3.36
Completeness [%]	98.7	95.4
Redundancy	5.2	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.2M NaNitrat 0.1M Bis Tris pH=6.5 20% PEG 3500, VAPOR DIFFUSION, SITTING DROP, temperature 292K