3JYH
Human dipeptidyl peptidase DPP7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.216, 130.215, 124.436 |
| Unit cell angles | 90.00, 102.36, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.190 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.346 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.220 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 127772 | |
| <I/σ(I)> | 25.9 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | 2M Ammonium sulfate, 0.2M Sodium acetate, 0.1M Hepes, 5% MPD, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
