3JYG
Crystal structure of uncharacterized protein WS1659 from Wolinella succinogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.838, 80.949, 104.614 |
| Unit cell angles | 90.00, 113.24, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23300 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.638 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.112 | 0.445 |
| Number of reflections | 106182 | |
| <I/σ(I)> | 7.6 | 2.9 |
| Completeness [%] | 99.3 | 93.3 |
| Redundancy | 6.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 5% Jeffamine ED-2001 pH 7.0, 1.1M Sodium Malonate pH 7.0, Vapor diffusion, Sitting drop, temperature 298K |
