3JY6
Crystal structure of LacI Transcriptional regulator from Lactobacillus brevis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-13 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.570, 109.328, 113.342 |
| Unit cell angles | 90.00, 95.17, 90.00 |
Refinement procedure
| Resolution | 40.410 - 1.970 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.26100 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.130 | 0.680 |
| Number of reflections | 65400 | |
| <I/σ(I)> | 7.5 | 3.9 |
| Completeness [%] | 95.1 | 80.7 |
| Redundancy | 7.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 298 | 0.2M Mg Cl2, 0.1M Bis Tris, 25% Peg 3350, 2M NaCl, Ethylene Glycol, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
