3JXV
Crystal Structure of the 3 FKBP domains of wheat FKBP73
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.168, 31.322, 96.716 |
| Unit cell angles | 90.00, 98.46, 90.00 |
Refinement procedure
| Resolution | 30.800 - 2.080 |
| R-factor | 0.25053 |
| Rwork | 0.248 |
| R-free | 0.28731 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.352 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.800 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.068 | 0.243 |
| Number of reflections | 25033 | |
| <I/σ(I)> | 54.3 | 6.9 |
| Completeness [%] | 99.3 | 94.3 |
| Redundancy | 7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 0.2M KNO3 0.1 Bis TrixPropane pH=8.5 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
