3JX8

Crystal structure of Putative lipid binding protein (YP_001304415.1) from Parabacteroides distasonis ATCC 8503 at 2.16 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97886
Spacegroup name	P 41 21 2
Unit cell lengths	201.880, 201.880, 202.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.207 - 2.160
R-factor	0.189
Rwork	0.187
R-free	0.21000
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.598
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.207	49.207	2.310
High resolution limit [Å]	2.160	4.780	2.160
Rmerge	0.093	0.045	0.709
Number of reflections	217900	20808	39505
<I/σ(I)>	8.91	21.4	2.02
Completeness [%]	96.7	97.4	91.5
Redundancy	3.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	2.30M ammonium sulfate, 0.1M Bicine pH 8.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K
2	VAPOR DIFFUSION, SITTING DROP	8.29	277	2.50M ammonium sulfate, 0.1M Bicine pH 8.29, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K