3JW6
Crystal structure of AcMNPV baculovirus polyhedra
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9789 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 103.183, 103.183, 103.183 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.840 - 2.300 |
| R-factor | 0.166 |
| Rwork | 0.161 |
| R-free | 0.21400 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.149 | 0.550 |
| Number of reflections | 8256 | |
| <I/σ(I)> | 6.2 | 3.8 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 6.6 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 300 | Natural intracellular crystals were directly purified from Sf21 cells infected by the AcMNPV baculovirus, pH 7, temperature 300K |
