3JW1
Crystal Structure of Bovine Pancreatic Ribonuclease Complexed with Uridine-5'-monophosphate at 1.60 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-04-29 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.730, 74.850, 50.520 |
| Unit cell angles | 90.00, 107.80, 90.00 |
Refinement procedure
| Resolution | 29.540 - 1.600 |
| R-factor | 0.203 |
| Rwork | 0.197 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.549 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.540 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.027 | 0.340 |
| Number of reflections | 25900 | |
| <I/σ(I)> | 16.8 | 2.4 |
| Completeness [%] | 91.2 | 64.2 |
| Redundancy | 2.61 | 2.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 0.1 M HEPES BUFFER; Reservoir of 25% PEG3350 in water; droplets were 5-10 mM in basic fuchsin, tobramycin, uridine-5'-monophosphate and 1 mM in ribonuclease A, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
